33342 熒光染料,bisBenzimide, HOE 33342, Hoechst 33342,
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產(chǎn)品名稱: 33342 熒光染料,bisBenzimide, HOE 33342, Hoechst 33342,
產(chǎn)品型號: Sigma B2261 25mg
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
33342 熒光染料,bisBenzimide, HOE 33342, Hoechst 33342,
33342 熒光染料,bisBenzimide, HOE 33342, Hoechst 33342,
的詳細介紹
33342 熒光染料,bisBenzimide, HOE 33342, Hoechst 33342,
33342 熒光染料
產(chǎn)品說明：Hoechst 33342，也稱bisBenzimide H 33342或HOE 33342。分子式為C27H28N6O·3HCl·3H2O，分子量為615.99，CAS Number 23491-52-3。
Hoechst 33342是一種可以穿透細胞膜的藍色熒光染料，對細胞的毒性較低。
Hoechst 33342染色常用于細胞凋亡檢測，染色后用熒光顯微鏡觀察或流式細胞儀檢測。Hoechst 33342也常用于普通的細胞核染色，或常規(guī)的DNA染色。
Hoechst 33342的*大激發(fā)波長為346nm，*大發(fā)射波長為460nm；Hoechst 33342和雙鏈DNA結(jié)合后，*大激發(fā)波長為350nm，*大發(fā)射波長為461nm。
溶解度： Hoechst 33342溶于水，溶解度可達20mg/ml。
用途：用于細胞核染色時，推薦的Hoechst 33342工作濃度為0.5-10μg/ml。
Sigma相關(guān)資料：
Sigma-Aldrich B2261 bisBenzimide H 33342 trihydrochloride
≥98% (HPLC and TLC)
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Synonym:
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bisBenzimide, HOE 33342, Hoechst 33342, 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride
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CAS Number:
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23491-52-3
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Linear Formula:
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C27H28N6O · 3HCl · xH2O
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Molecular Weight:
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561.93 (anhydrous basis)
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Beilstein Registry Number:
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1234011
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EC Number:
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245-691-1
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MDL number:
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MFCD00012678
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PubChem Substance ID:
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24891639
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Description
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Frequently Asked Questions
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Live Chat and Frequently Asked Questions are available for this Product.
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Application
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Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm
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Biochem/physiol Actions
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Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.
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Properties
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assay
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≥98% (HPLC and TLC)
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solubility
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H2O: 20 mg/mL
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phosphate buffers: precipitates
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suitability
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passes application test for fluorescence
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storage temp.
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−20°C
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Safety
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Hazard Codes
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Xn
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Risk Statements
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22-37/38
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Safety Statements
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36/37
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WGK Germany
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1
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品名
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產(chǎn)地
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貨號
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規(guī)格
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單價
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備注
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HoechstNo. 33342 熒光染料
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Sigma
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B2261
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25mg
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詢價
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現(xiàn)貨
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